Inhibition of PHGDH, ROS, and PARP in Cancer Cells

The PHGDH inhibitor CBR-5884, the ROS inhibitor N-acetylcysteine, and the PARP inhibitor olaparib were purchased from MedChemExpress. DMSO was used as control group in cell lines. Through literature reviewing, we used 5 mM NAC treated for 4 h to study its effect [36 (link), 37 (link)]. The cell lines (A2780, OVCAR3, and ES-2) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). A2780 and OVCAR3 were cultured with RPMI 1640 medium (Procell, Wuhan, China) containing 10% FBS (Procell, Wuhan, China), while ES-2 was cultured used McCoy's 5A (Procell, Wuhan, China) containing 10% FBS (Procell, Wuhan, China) in incubator. The incubator purchased from PUHE Biotechnology Co., Ltd. (Wuxi, China) was set at 37 °C, 5% CO2, and 1% O2 with enough humidity.