Dual Luciferase Assay for Gene Expression
Corresponding Organization : National Cancer Center
Other organizations : Yonsei University
Variable analysis
- Transfection with NC siRNA or siFAM188B
- Luciferase activity (pGL2-p21-luc and pGL4.70hRluc)
- Time points (24 and 48 h)
- Dual luciferase assay protocol (Promega)
- Measurement using Victor3 reader (Perkin Elmer)
- Positive control: pGL4.70hRluc plasmid
- Negative control: NC siRNA
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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