Cuprizone ingestion results in a reproducible pattern of extensive corpus callosum demyelination (12, 13 (link)). Cuprizone treatment was started at 8 weeks of age and only male mice were used. Cuprizone (0.2% (w/w), finely powdered oxalic bis(cyclohexylidenehydrazide) (Sigma-Aldrich, St. Louis, MO), was thoroughly mixed into chow (diet TD.01453; Harlan Teklad, Madison, WI), which was available ad libitum. Mice were maintained on the cuprizone diet until perfused for analysis or returned to normal chow after 6 weeks or 12 weeks of cuprizone ingestion.
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Cuprizone-Induced Corpus Callosum Demyelination
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Other organizations : Uniformed Services University of the Health Sciences
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Variable analysis
independent variables
- Cuprizone treatment duration (6 weeks or 12 weeks)
- Corpus callosum demyelination
- Age of mice (started at 8 weeks)
- Sex of mice (only male mice used)
- Cuprizone concentration (0.2% (w/w))
- Diet (Cuprizone mixed into chow, available ad libitum)
- Positive control: Mice maintained on normal chow after 6 weeks or 12 weeks of cuprizone ingestion
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