Annotated Mango FT-like Sequence Isolation

A previously published annotated sequence of MiFT from mango cv. Irwin (AB671587.1), and two annotated sequences of MiTFL1s from mango cv. Alphonso (KF258590 and KU206290), were utilized in this study. We also made use of an additional sequence annotation, putatively encoding for an FT-like gene (mango_rep_c5502), which was identified in a search conducted against ‘Tommy Atkins’ and ‘Keitt’ mango transcriptomes [71 (link)]. The four corresponding ‘Shelly’ cDNAs were isolated by real-time qPCR using pairs of end-to-end primers, designed within the 5′ and 3′ UTRs of each sequence annotation (see Table S1), and cDNA synthesized from ‘Shelly’ tissues as the template (a mixture of samples collected from leaves and buds at different time points). The obtained PCR products were ligated into the CloneJET vector (Fermentas, Vilnius, Lithuania), sequenced (Hy-labs Laboratories, Rehovot, Israel), and further used as templates to generate constructs for Arabidopsis transformation purposes.