Human umbilical cord vein endothelial cells (HUVECs) were obtained as previously described with minor modifications[14 (link)]. Briefly, umbilical cord veins were washed with phosphate buffered saline (PBS) and subjected to collagenase digestion. Resultant HUVECs were seeded onto T-25 culture flasks and maintained in endothelial basal medium EBM-Plus (Lonza, MD). All of the cells used in this study were from passages 1 to 4.
Protocol detail
Isolation and Culture of HUVECs
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Cells
Collagenase
Cord
Digestion
Endothelial
Human umbilical vein endothelial cells
Phosphate
Saline
Umbilical cord
Umbilical veins
Veins
Corresponding Organization : Michael E. DeBakey VA Medical Center
Other organizations : National Cancer Institute
Top 5 similar protocols
Variable analysis
independent variables
- Collagenase digestion
- Resultant HUVECs
- Passages 1 to 4 of HUVECs
- No positive or negative controls were explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!