Evaluating Fibrosis Progression in Mast Cell-Deficient Mice

We measured fibrosis progression and collagen deposition from both WT and Kit^W-sh mice with sham or BDL surgery. Fast Green/Sirius Red, Masson’s Trichrome and hydroxyproline content were assessed in liver sections and snap liver, respectively (29 (link)). In total liver from all groups, we measured the following fibrotic markers by real-time PCR: α-smooth muscle actin (α-SMA), collagen type-1a and fibronectin-1 (29 (link)). Since TGF-β1 is a known regulator of fibrosis (11 (link)) we evaluated the expression of TGF-β1 in total liver by real-time PCR and the secretion of TGF-β1 by EIA in serum from these mice (19 ). We also evaluated the effects of mast cell deficiency on HSC activation by immunofluorescence for synaptophysin-9 (SYP-9) (32 (link)). Livers were co-stained with CK-19 to also visualize bile ducts.

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Hargrove L., Kennedy L., Demieville J., Jones H., Meng F., DeMorrow S., Karstens W., Madeka T., Greene J J.r, & Francis H. (2017). BDL-induced biliary hyperplasia, hepatic injury and fibrosis are reduced in mast cell deficient Kitw-sh mice. Hepatology (Baltimore, Md.), 65(6), 1991-2004.

Publication 2017

Actin Bile ducts Ck 19 Collagen Fast green Fibronectin 1 Fibrosis Hydroxyproline Immunofluorescence Liver groups Livers Mast cell Mice Progression Real time pcr Secretion Serum Smooth muscle Surgery Synaptophysin Tgf β1

Corresponding Organization : Texas A&M Health Science Center

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Protocol cited in 11 other protocols

Variable analysis

independent variables

Mouse genotype (WT vs. Kit^W-sh)
Surgery type (sham vs. BDL)

dependent variables

Fibrosis progression
Collagen deposition
Expression of fibrotic markers (α-SMA, collagen type-1a, fibronectin-1)
Expression of TGF-β1 in total liver
Secretion of TGF-β1 in serum
HSC activation (synaptophysin-9 expression)

control variables

Liver sections and snap liver samples

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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