This was an ongoing rolling longitudinal study designed to evaluate immunity to malaria in children and is described elsewhere40 (link). Within this cohort, children were followed to 8 years of age with weekly follow-ups and annual cross-sectional surveys during which anthropometry measurements were made and blood samples were collected. Serum ferritin (Chemiluminescent Microparticle Immunoassay, Abbott Architect), hepcidin (DRG Hepcidin 25 (bioactive) High Sensitive ELISA kit (DRG International)), CRP (Multigent CRP Vario assay, Abbott Architect), hemoglobin (Coulter analyzer, Beckman Coulter) and malaria parasitemia (Giemsa-stained thick and thin blood films) were measured from blood samples collected at a single cross-sectional survey based on the availability of plasma samples archived at 80°C. Genotyping of hemoglobin types and α-thalassemia was conducted by PCR35 (link),36 (link) using DNA extracted with the Qiagen DNA Blood Mini kit (Qiagen).
Corresponding Organization : Kenya Medical Research Institute
Other organizations :
Wellcome Centre for Human Genetics, University of Oxford, University of Bristol, Medical Research Council, London School of Hygiene & Tropical Medicine, African Population and Health Research Center, Oxford University Hospitals NHS Trust, Uganda Virus Research Institute, University of Mississippi Medical Center, Jackson Memorial Hospital, University of North Carolina at Chapel Hill, South African Medical Research Council, University of the Witwatersrand, Groupe de Recherche et d’Action sur le Foncier, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Center for Infectious Disease Research, University of Ghana, Wellcome Sanger Institute, MRC Unit the Gambia, WorldFish, Helen Keller International, University of California, Davis, South Australian Health and Medical Research Institute, University of British Columbia, Emory University
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