PP2A immunoprecipitates were washed with phosphatase assay buffer and incubated with a PP2A-specific threonine phosphopeptide substrate. Phosphate release and PP2A activity was assessed using a malachite green assay kit (Millipore-Sigma, Burlington, MA)(Davuluri et al., 2021 (link)).
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PP2A Activity Measurement Using Malachite Green
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Corresponding Organization : Cleveland Clinic Lerner College of Medicine
Other organizations : Maastricht University, Case Western Reserve University
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Variable analysis
independent variables
- Incubation of PP2A immunoprecipitates with a PP2A-specific threonine phosphopeptide substrate
- Phosphate release
- PP2A activity
- Phosphatase assay buffer used to wash PP2A immunoprecipitates
- Positive control: None mentioned
- Negative control: None mentioned
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