Induced Myogenic Cells from hiPSCs

201B7, 409B2, and 454E2 iPSCs were provided by S. Yamanaka at Kyoto University. 201B7 was generated from a healthy donor using retroviral vectors [20 (link)]. 409B2 and 454E2 were integration-free hiPS clones generated from healthy donors using episomal vectors [21 (link)]. hiPSCs were cultured on iMatrix-511 (Nippi)-coated 6-well plates in StemFit AK02N medium (Ajinomoto) supplemented with penicillin/streptomycin/amphotericin B (1% v/v) (FujiFilm) as described previously [37 (link), 38 (link)]. Myogenic cells were induced from hiPSCs as described [37 (link), 38 (link)]. After floating culture, cells were induced to differentiate into myogenic cells on collagen type I-coated 10-cm dishes (Iwaki) in 10% FBS (Gibco)/DMEM (FujiFilm) in the presence of 1 μM SB431542 (Wako) for one week. The induced cells were then collected and incubated with ERBB3-APC (1B4C3, BioLegend), CD57(HNK-1)-PE (clone TB03, Miltenyi Biotec), and CD271-BB515 (C40-1457, BD Pharmingen). Myogenic cells were sorted as the CD57(-) CD271(+) ERBB3(+) fraction [37 (link), 38 (link)].

Free full text: Click here

Elhussieny A., Nogami K., Sakai-Takemura F., Maruyama Y., Takemura N., Soliman W.T., Takeda S, & Miyagoe-Suzuki Y. (2021). Mesenchymal stem cells derived from human induced pluripotent stem cells improve the engraftment of myogenic cells by secreting urokinase-type plasminogen activator receptor (uPAR). Stem Cell Research & Therapy, 12, 532.

Publication 2021

StemFit AK02N medium collagen type I-coated 10-cm dishes SB431542 CD57(HNK-1)-PE (clone TB03,

Amphotericin Cd271 Cells Clone Collagen type i Dishes Donor Donors Episomal Hips Hipscs Hnk 1 Ipscs Myogenic Penicillin b Retroviral Sb431542 Streptomycin Vectors

Corresponding Organization :

Other organizations : National Center of Neurology and Psychiatry, Minia University

Top 5 similar protocols

Variable analysis

independent variables

Retroviral vector
Episomal vector

dependent variables

Myogenic cells induced from hiPSCs

control variables

IMatrix-511 (Nippi)-coated 6-well plates
StemFit AK02N medium (Ajinomoto) supplemented with penicillin/streptomycin/amphotericin B (1% v/v) (FujiFilm)
Collagen type I-coated 10-cm dishes (Iwaki)
10% FBS (Gibco)/DMEM (FujiFilm) in the presence of 1 μM SB431542 (Wako)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!