Exploring OPN-R3 Aptamer Interactions

The pharmacologic properties and sequences of OPN-R3 aptamer (APT) and mutant aptamer (MuAPT) have been previously published.^{28 (link)}Recombinant human OPN and TGF-β1 proteins (R&D Systems, Minneapolis, MN); αvβ3 integrin and CD44 Abs (Santa Cruz Biotechnology, Santa Cruz, CA); Arg-Gly-Asp (RGD), Arg-Gly-Glu (RGE) (Sigma-Aldrich, St Louis, MO); SB431542 a TGF- β1 type I receptor inhibitor (EMD Millipore, Billerica, MA); TGF-β1 type II receptor shRNA (Santa Cruz Biotechnology); MZF1 siRNA (Life Technologies, Carlsbad, CA); and Control siRNA^{81 (link)}: UAAGGCUAUGAAGAGAUAC were all obtained.

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Weber C.E., Kothari A.N., Wai P.Y., Li N.Y., Driver J., Zapf M.A., Franzen C.A., Gupta G.N., Osipo C., Zlobin A., Syn W.K., Zhang J., Kuo P.C, & Mi Z. (2014). Osteopontin Mediates an MZF1-TGF-β1-Dependent Transformation of Mesenchymal Stem Cells into Cancer Associated Fibroblasts in Breast Cancer. Oncogene, 34(37), 4821-4833.

Publication 2014

Recombinant human OPN and TGF-β1 proteins αvβ3 integrin and CD44 Abs SB431542 a TGF- β1 type I receptor inhibitor TGF-β1 type II receptor shRNA MZF1 siRNA

Arg gly glu Cd44 Human Integrin Proteins Sb431542 Shrna Sirna Tgf β1

Corresponding Organization :

Other organizations : Loyola University Medical Center, Loyola University Chicago

Top 5 similar protocols

Variable analysis

independent variables

OPN-R3 aptamer (APT)
Mutant aptamer (MuAPT)
Recombinant human OPN
Recombinant human TGF-β1
Arg-Gly-Asp (RGD)
Arg-Gly-Glu (RGE)
SB431542 (TGF-β1 type I receptor inhibitor)
TGF-β1 type II receptor shRNA
MZF1 siRNA
Control siRNA

dependent variables

Pharmacologic properties and sequences of OPN-R3 aptamer (APT) and mutant aptamer (MuAPT)

control variables

αvβ3 integrin
CD44 antibodies

positive controls

Recombinant human OPN
Recombinant human TGF-β1

negative controls

Arg-Gly-Glu (RGE)
Control siRNA

Annotations

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