The antioxidant activities ABTS+ (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate), DPPH (2,2-diphenyl-1-picrylhydrazyl), and FRAP (ferric reduction antioxidant potency) were determined by UV-VIS spectrophotometry (Shimadzu, Japan) according to the protocols described by Formisano et al. (2021) (link). Results were expressed as mmol Trolox equivalents kg–1 dw. Phenolic Concentration Determination
One hundred milligrams of freeze-dried basil were used to quantify and determine polyphenols using a UHPLC system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a thermo-stated column (T=25°C, 100 × 2.1 mm, Kinetex 1.7 µm biphenyl, Phenomenex, Torrance, CA, USA) and a quaternary pump (Ultimate 3000, Dionex, Sunnyvale, CA, USA). Mass spectrometry analysis was facilitated by a Q Exactive Orbitrap LC-MS/MS system (Thermo Fisher Scientific, Waltham, MA, USA). Phenolic compound sampling was performed according to the protocol detailed by Pannico et al. (2020) (link). The accuracy and calibration of the instruments used were set and checked using a mixture of reference standards (Thermo Fisher Scientific, Waltham, MA, USA). Data processing and analysis were performed using Xcalibur software, version 3.0.63 (Thermo Fisher Scientific, Waltham, MA, USA), and the results were expressed as µg g–1 dw.
One hundred milligrams of freeze-dried basil were used to quantify and determine polyphenols using a UHPLC system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a thermo-stated column (T=25°C, 100 × 2.1 mm, Kinetex 1.7 µm biphenyl, Phenomenex, Torrance, CA, USA) and a quaternary pump (Ultimate 3000, Dionex, Sunnyvale, CA, USA). Mass spectrometry analysis was facilitated by a Q Exactive Orbitrap LC-MS/MS system (Thermo Fisher Scientific, Waltham, MA, USA). Phenolic compound sampling was performed according to the protocol detailed by Pannico et al. (2020) (link). The accuracy and calibration of the instruments used were set and checked using a mixture of reference standards (Thermo Fisher Scientific, Waltham, MA, USA). Data processing and analysis were performed using Xcalibur software, version 3.0.63 (Thermo Fisher Scientific, Waltham, MA, USA), and the results were expressed as µg g–1 dw.
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