The histological evaluation was performed at 4 weeks post surgeries. The rats were anesthetized with isoflurane and transcardially perfused with 4% paraformaldehyde in PBS. Spinal cord tissue was cut into paraffin sagittal sections of 7 μm thickness. After the paraffin sections were prepared, the paraffin sections were stained with hematoxylin-eosin (Solarbio, China), as described previously [27 (link)]. Finally, the stained sections were observed under a microscope (Nikon, Japan).
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Histological Evaluation of Spinal Cord Tissue
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Other organizations : Tianjin Medical University General Hospital
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Variable analysis
independent variables
- Time post surgeries (4 weeks)
- Histological evaluation of spinal cord tissue
- Anesthesia with isoflurane
- Transcardial perfusion with 4% paraformaldehyde in PBS
- Paraffin sectioning of spinal cord tissue (7 μm thickness)
- Hematoxylin-eosin staining of paraffin sections
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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