SARS-CoV-2 Antibody Detection Techniques

CPT tubes were processed for plasma up to 16 hours after collection, but typically within 8 hours of collection. Plasma collected from participants at in-person study visits and serum self-collected by participants via Tasso devices were both tested via ELISA, using the receptor binding domain (RBD) of the SARS-CoV-2 spike protein to detect total SARS-CoV-2 immunoglobulin (Ig) in plasma [23 (link)]. Specimens were also tested via the Abbott Architect Immunoassay, using the nucleocapsid protein to detect total SARS-CoV-2 IgG in plasma [24 (link)].

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Miller E.M., Law E.A., Ajeen R., Karasik J., Mendoza C., Abernathy H., Garrett H., King E., Wallace J., Zelek M., Edwards J.K., Xiong K., Beatty C., Fleischauer A.T., Ciccone E.J., Shook-Sa B.E., Aiello A.E, & Boyce R.M. (2021). SARS-CoV-2 infection in central North Carolina: Protocol for a population-based longitudinal cohort study and preliminary participant results. PLoS ONE, 16(10), e0259070.

Publication 2021

Architect Immunoassay

Binding protein Devices Elisa Immunoassay Immunoglobulin Nucleocapsid protein Plasma Protein domain Sars cov 2 Serum

Corresponding Organization : NC Department of Health and Human Services

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Variable analysis

independent variables

Time of plasma processing (up to 16 hours after collection, typically within 8 hours)

dependent variables

Plasma SARS-CoV-2 total immunoglobulin (Ig) levels detected by ELISA
Plasma SARS-CoV-2 total IgG levels detected by Abbott Architect Immunoassay

control variables

Specimen collection method (in-person study visits and self-collected via Tasso devices)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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