Quantitative RNA Expression Analysis

RNA was isolated from muscle tissues or from cultured cells with TriPure reagent (Sigma-Aldrich). RT-qPCR primers for mouse cyclophilin, NLRP3 and ASC were used as previously reported (3 (link)). New mouse primer sequences were transforming growth factor beta (TGFβ) (Table S2). RT-qPCR primers for human TATA box-binding protein (TBP), TNFα, and IL-1β, were also similar to those previously reported (22 (link)). New human primer sequences were IL-18 (Table S2). Threshold cycles (Ct) were measured in duplicate.

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Dubuisson N., Davis-López de Carrizosa M.A., Versele R., Selvais C.M., Noel L., Van den Bergh P.Y., Brichard S.M, & Abou-Samra M. (2022). Inhibiting the inflammasome with MCC950 counteracts muscle pyroptosis and improves Duchenne muscular dystrophy. Frontiers in Immunology, 13, 1049076.

Publication 2022

TriPure reagent

Cultured cells Cyclophilin Human Human tata box binding protein Il 18 Il 1β Mouse Muscle tissues Primer Tgfβ Tnfα

Corresponding Organization : Cliniques Universitaires Saint-Luc

Other organizations : Universidad de Sevilla

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Variable analysis

independent variables

RNA isolation method (TriPure reagent)

dependent variables

Expression levels of cyclophilin, NLRP3, ASC, TGFβ, TBP, TNFα, IL-1β, IL-18 (measured by RT-qPCR)

control variables

Mouse and human cell/tissue samples

controls

Threshold cycles (Ct) were measured in duplicate

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