Establishing Lung Mesenchymal Cell Lines

Six primary lung mesenchymal cell lines were established from 6 individual patients (3 male and 3 female; average age, 63), fulfilling diagnostic criteria for IPF including a pathological diagnosis of usual interstitial pneumonia (1 (link)) at the time of lung transplantation. Control MPCs were derived from lung tissue (2 male and 4 female; average age, 57) obtained by video-assisted thoracoscopic surgery (VATS) biopsy or lobectomy and uninvolved from the primary disease process (adenocarcinoma, n = 1; carcinoid tumor, n = 1; synovial sarcoma, n = 1; leiomyosarcoma, n = 1; bronchiectasis, n = 1; and normal lung tissue, n = 1). Cell lines were derived from lungs, characterized as mesenchymal cells, and cultivated as previously described (70 (link)).