Isolation and Maintenance of HLSCs

HLSCs were isolated and maintained in a culture as previously described [34 (link),47 (link),48 (link)]. HLSCs were plated at a density of 2.5 × 10⁵ cells per flask (T75, Corning, VWR International, Milano, Italy) and maintained in the presence of Minimal Essential Medium (α-MEM, Lonza, Basel, Switzerland) with 10% Fetal Calf Serum (Gibco/Cambrex, Invitrogen, Carlsbad, CA, USA), specific growth factors (10 ng/mL of human recombinant Epidermal Growth Factor and of human recombinant Fibroblast Growth Factor basic, Miltenyi, Bergisch Gladbach, Germany), 2nM L-Glutamine (Lonza), and antibiotics (100U/mL of Penicillin/Streptomycin, Sigma-Aldrich, St. Louis, MO, USA).

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Bruno S., Chiabotto G., Cedrino M., Ceccotti E., Pasquino C., De Rosa S., Grange C., Tritta S, & Camussi G. (2022). Extracellular Vesicles Derived from Human Liver Stem Cells Attenuate Chronic Kidney Disease Development in an In Vivo Experimental Model of Renal Ischemia and Reperfusion Injury. International Journal of Molecular Sciences, 23(3), 1485.

Publication 2022

human recombinant Epidermal Growth Factor human recombinant Fibroblast Growth Factor basic L-Glutamine Penicillin/Streptomycin

Antibiotics Epidermal growth factor Fetal calf serum Glutamine Growth factors Human Penicillin Recombinant human basic fibroblast growth factor Streptomycin

Corresponding Organization : University of Turin

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Variable analysis

independent variables

Plating density of HLSCs (2.5 × 10^5 cells per flask)

dependent variables

Not explicitly mentioned

control variables

Culture medium (Minimal Essential Medium (α-MEM) with 10% Fetal Calf Serum, specific growth factors (10 ng/mL of human recombinant Epidermal Growth Factor and of human recombinant Fibroblast Growth Factor basic), 2nM L-Glutamine, and antibiotics (100U/mL of Penicillin/Streptomycin))
Culture vessel (T75 flask, Corning, VWR International, Milano, Italy)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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