Identifying miR-146b-5p Regulatory Targets in ccRCC

Expression data of ccRCC mRNAs (normal: 72, tumor: 539) and mature miRNAs (normal: 71, tumor: 545) were retrieved from TCGA database (https://portal.gdc.cancer.gov/). The R package “edgeR” (log|FC|> 1.5, FDR < 0.05) was employed to compare the expression of miRNA and mRNA in the normal and tumor groups (log|FC|> 1.5, FDR < 0.05). And we utilized R package “survival” to examine association between miR-146b-5p and the prognoses of ccRCC patients. To determine miRNA downstream regulatory target genes, we used the miRDB (http://mirdb.org/), TargetScan (http://www.targetscan.org/vert_72/), starBase (http://starbase.sysu.edu.cn/) and mirDIP (http://ophid.utoronto.ca/mirDIP/index.jsp#r) databases. Differential mRNAs with targeted binding sites to target miRNAs were screened, which were intersected with differentially down-regulated mRNAs. Then target genes were finally determined using correlation analysis. GSEA software was applied to perform a KEGG analysis.

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Tang M, & Xiong T. (2023). MiR-146b-5p/SEMA3G regulates epithelial-mesenchymal transition in clear cell renal cell carcinoma. Cell Division, 18, 4.

Publication 2023

Binding sites Cancer Down Genes Mirnas Mrnas Patients Prognoses Regulatory genes Tumor Vert

Corresponding Organization : Rongcheng City People's Hospital

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Variable analysis

independent variables

Expression data of ccRCC mRNAs
Expression data of mature miRNAs

dependent variables

Differentially expressed mRNAs and miRNAs between normal and tumor groups (log|FC|> 1.5, FDR < 0.05)
Associations between miR-146b-5p and prognoses of ccRCC patients

control variables

Normal and tumor groups of ccRCC samples

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