Immunostaining of Ovaries and Embryos
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Corresponding Organization :
Other organizations : University of Rochester, University of Rochester Medical Center
Variable analysis
- Duration of fixation in 10% Formaldehyde and 0.2% Tween in Phosphate Buffered Saline (PBS‐T) for ovaries
- Duration of fixation at the interface of 37% Formaldehyde and 100% Heptane for embryos
- Staining of ovaries and embryos
- Phosphate Buffered Saline (PBS)
- PBS‐T
- Blocking solution (10% Bovine Serum Albumin in PBS)
- Duration of primary and secondary immunostainings (at least 3 h in PBS‐T)
- Number of washes in PBS‐T (three washes of about 10 min each)
- Concentration of primary and secondary antibodies (1:150)
- Positive control: Not specified
- Negative control: Not specified
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