Retroviral NF2 Gene Overexpression

The pMXs-NF2-IRES-GFP construct was generated by inserting NF2 cDNA (Open Biosystems, MHS1011-202832561) into pMXs-IRES-GFP retroviral vector (Cell Biolabs). Retroviral packaging, infection, and fluorescence-activated cell sorting (FACS) were performed as previously described (22 (link)).

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Shapiro I.M., Kolev V.N., Vidal C.M., Kadariya Y., Ring J.E., Wright Q., Weaver D.T., Menges C., Padval M., McClatchey A.I., Xu Q., Testa J.R, & Pachter J.A. (2014). Merlin deficiency predicts for FAK inhibitor sensitivity: A synthetic lethal relationship. Science translational medicine, 6(237), 237ra68.

Publication 2014

pMXs-IRES-GFP retroviral vector

Cdna Cell Infection Ires Retroviral Vector

Corresponding Organization :

Other organizations : Verastem (United States), Fox Chase Cancer Center, Center for Cancer Research, Harvard University, Massachusetts General Hospital

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Variable analysis

independent variables

Insertion of NF2 cDNA into pMXs-IRES-GFP retroviral vector

dependent variables

Retroviral packaging, infection, and fluorescence-activated cell sorting (FACS)

control variables

Retroviral packaging, infection, and fluorescence-activated cell sorting (FACS) procedures performed as previously described (22)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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