STING Pathway Activation and Inhibition

2′3′-cGAMP (tlrl-nacga23-02) was purchased from InvivoGen. Ibrutinib (BTK inhibitor; no. S2680), PF-562271 (FAK inhibitor; no. S2890), PP2 (Src inhibitor; no. S7008), saracatinib (Fyn inhibitor; no. S1006), amuvatinib (c-Kit inhibitor; no. S1244), ruxolitinib (Jak1/2 inhibitor; no. S1378), alectinib (ALK inhibitor; no. S2762), gefitinib (EGFR inhibitor; no. S1025), AZD9291 (EGFR inhibitor; no. S7297), and R406 (Syk inhibitor; no. S2194) were obtained from Selleck. Polyethyleneimine (PEI; no. 195444) was purchased from Mpbio, and the transfection procedure was as previously described (16 (link), 33 (link)). Lipofectamine 2000 (no. 11668019) was obtained from Invitrogen. Antibodies against EGFR (no. 4267), GFP (no. 2956), phospho-Syk (Tyr525/526) (no. 2710), Syk (no. 13198), tubulin (no. 2146), and actin (no. 3700) were from Cell Signaling, and hemagglutinin (HA) antibody (ab18181) was purchased from Abcam. STING antibody (A3575) and GST (AE001) antibodies were obtained from Abclonal Technology.

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Wang C., Sharma N., Veleeparambil M., Kessler P.M., Willard B, & Sen G.C. (2021). STING-Mediated Interferon Induction by Herpes Simplex Virus 1 Requires the Protein Tyrosine Kinase Syk. mBio, 12(6), e03228-21.

Publication 2021

2′3′-cGAMP (tlrl-nacga23-02) Ibrutinib PF-562271 saracatinib amuvatinib ruxolitinib alectinib gefitinib AZD9291 Polyethyleneimine Lipofectamine 2000 phospho-Syk (Tyr525/526)

Actin Alectinib Amuvatinib Antibodies Antibody Azd9291 C kit Cgamp Egfr Gefitinib Hemagglutinin Ibrutinib Jak1 Lipofectamine 2000 Polyethyleneimine Ruxolitinib Saracatinib Transfection Tubulin

Corresponding Organization : Cleveland Clinic Lerner College of Medicine

Top 5 similar protocols

Variable analysis

independent variables

2'3'-cGAMP (tlrl-nacga23-02)
Ibrutinib (BTK inhibitor; no. S2680)
PF-562271 (FAK inhibitor; no. S2890)
PP2 (Src inhibitor; no. S7008)
Saracatinib (Fyn inhibitor; no. S1006)
Amuvatinib (c-Kit inhibitor; no. S1244)
Ruxolitinib (Jak1/2 inhibitor; no. S1378)
Alectinib (ALK inhibitor; no. S2762)
Gefitinib (EGFR inhibitor; no. S1025)
AZD9291 (EGFR inhibitor; no. S7297)
R406 (Syk inhibitor; no. S2194)
Polyethyleneimine (PEI; no. 195444)
Lipofectamine 2000 (no. 11668019)

dependent variables

Phospho-Syk (Tyr525/526)
Tubulin
Actin
STING

control variables

Transfection procedure as previously described (16, 33)

Annotations

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