Pure
((2S,3S,4S)-3-(carboxymethyl)-4-(prop-1-en-2-yl)pyrrolidine-2-carboxylic
acid)(−)-kainic acid (99.8%) andl -valine 99.9% were
purchased from Sigma-Aldrich Ltd. Those components were dissolved
in deionized water (DIW 18 MΩ) and mixed with vortex for 30
min to ensure saturation. The solutions were filtered in 0.22 μm
vacuum filters (Corning) and then poured into a 2 in. glass Petri
dish.
The crystals start forming a small needle after several
minutes; then, it is left to slowly grow for 48–72 h. To achieve
1–2 cm crystals, another growth step was done by placing samples
into a saturated solution, followed by slow evaporation for another
72 h. The crystals were then placed on a fiber-free paper to dry before
further characterizations and slightly polished.
((2S,3S,4S)-3-(carboxymethyl)-4-(prop-1-en-2-yl)pyrrolidine-2-carboxylic
acid)(−)-kainic acid (99.8%) and
purchased from Sigma-Aldrich Ltd. Those components were dissolved
in deionized water (DIW 18 MΩ) and mixed with vortex for 30
min to ensure saturation. The solutions were filtered in 0.22 μm
vacuum filters (Corning) and then poured into a 2 in. glass Petri
dish.
The crystals start forming a small needle after several
minutes; then, it is left to slowly grow for 48–72 h. To achieve
1–2 cm crystals, another growth step was done by placing samples
into a saturated solution, followed by slow evaporation for another
72 h. The crystals were then placed on a fiber-free paper to dry before
further characterizations and slightly polished.
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