Liver Metabolism and Antioxidant Bioassay

Liver tissues were homogenized with physiological saline, centrifuged twice at 3500 r/min for 10 min each time, and the protein concentrations were determined using a bicinchoninic acid protein assay kit (#23227; Thermo Fisher Scientific). The levels of high-density lipoprotein cholesterol (HDL-C, #KT2827-A), total cholesterol (#KT30043-A), low-density lipoprotein cholesterol (LDL-C, #KT2797-A), aspartate aminotransferase (AST, #KT2856-A), glycosylated hemoglobin A1c (GHbA1c, #KT2511-A), insulin (#KT2579-A), adiponectin (#KT2547-A), resistin (#KT2552-A), ROS (#KT9261-A), malondialdehyde (MDA, #KT9264-A), glutathione peroxidase (GSH-Px, #KT2755-A), and catalase (CAT, #KT2833-A) were determined using enzyme-linked immunosorbent assay kits (Jiangsu Kete Biotechnology Co., Ltd., Jiangsu, China) according to the manufacturer’s instruction manuals.

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Li L., Zhai S., Wang R., Kong F., Yang A., Wang C., Yu H., Li Y, & Wang D. (2023). Anti-Obesity Effect of Auricularia delicate Involves Intestinal-Microbiota-Mediated Oxidative Stress Regulation in High-Fat-Diet-Fed Mice. Nutrients, 15(4), 872.

Publication 2023

bicinchoninic acid protein assay kit

Adiponectin Aspartate aminotransferase Assay Bicinchoninic acid Catalase Cholesterol Enzyme linked immunosorbent assay Glutathione peroxidase Glycosylated hemoglobin a1c High cholesterol levels Insulin Lipoprotein Liver Low density lipoprotein cholesterol Malondialdehyde Physiological Protein Resistin Saline Tissues

Corresponding Organization :

Other organizations : Jilin Agricultural University, Jilin University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

High-density lipoprotein cholesterol (HDL-C)
Total cholesterol
Low-density lipoprotein cholesterol (LDL-C)
Aspartate aminotransferase (AST)
Glycosylated hemoglobin A1c (GHbA1c)
Insulin
Adiponectin
Resistin
Reactive oxygen species (ROS)
Malondialdehyde (MDA)
Glutathione peroxidase (GSH-Px)
Catalase (CAT)

control variables

Protein concentrations were determined using a bicinchoninic acid protein assay kit (#23227; Thermo Fisher Scientific)

positive controls

None mentioned

negative controls

None mentioned

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