Cell line culture for arthritis and skin research

Chondrosarcoma (SW1353, HTB-94) and normal dermal fibroblast (BJ, CRL-2522) cell lines were acquired from American Type Culture Collection. SW1353 and BJ cell lines were utilized for their relevance as a disease-related model for arthritis and skin manifestations, respectively [36 (link),37 (link)]. SW1353 cells were grown in Leibovitz’s L-15 media (Sigma), supplemented with 10% fetal bovine serum (Gibco, Paisley, UK), 2 mM l-glutamine (Gibco), and 100 IU/mL Penicillin/0.2 mg/mL streptomycin (Gibco) antibiotic cocktail, and incubated at +37 °C with 100% air. BJ cells were grown in Eagle’s minimum essential media (Sigma), with the above-mentioned supplements and an additional 1 mM sodium pyruvate (Gibco), and incubated at +37 °C, 5% CO₂.