Maternal blood was collected by trained research staff at the 2nd trimester visit (12 – 34 weeks; n = 894), 3rd trimester visit (26 – 38 weeks; n = 745), and at birth (maternal n = 732; cord n = 515) and stored at −20°C. Mn was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS/MS) as previously described for Pb with the appropriate quality controls (20 (link)). In brief, blood was digested in HNO3 and 30% H2O2 and analyzed via ICP-MS/MS on an Agilent 8800 ICP Triple Quad (ICP-QQQ) instrument (Agilent technologies, Inc., Delaware, USA) with Indium as the internal standard. The quantitation limit of detection (LOD) was 0.02 – 0.08 μg/dL with no values below the LOD.
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Maternal-Cord Blood Manganese Levels
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Corresponding Organization :
Other organizations : Columbia University, Instituto Nacional de Perinatología, Instituto Nacional de Salud Pública, Icahn School of Medicine at Mount Sinai, Boston Children's Hospital, Harvard University
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Variable analysis
independent variables
- Maternal blood collection time points: 2nd trimester (12 – 34 weeks), 3rd trimester (26 – 38 weeks), and at birth
- Manganese (Mn) levels in maternal blood and cord blood
- Blood samples were stored at -20°C
- Mn was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS/MS)
- Indium was used as the internal standard
- Quantitation limit of detection (LOD) was 0.02 – 0.08 μg/dL, and there were no values below the LOD
- Appropriate quality controls were used, as previously described for Pb analysis
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