Western blotting was performed according to the standard protocol (21 (link)) with the following antibodies: EPB41L3 (1:2,000; Abcam, Cambridge, MA, USA), caspase-8 (1:1,000), caspase-9 (1:1,000), caspase-3 (1:1,000), CyclinA (1:2,000), CyclinD (1:1,000), CyclinE (1:1,000) (all from Cell Signaling Technology, Inc., Danvers, MA, USA), CyclinB1 (1:1,000), Cyclin-dependent kinase 1 (CDK1, also known as Cdc2; 1:1,000) (both from ProteinTech Wuhan Sanying Biotechnology, Wuhan, China). GAPDH (1:8,000; ProteinTech Wuhan Sanying Biotechnology) was used as loading control.
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Western Blotting Antibodies and Parameters
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Corresponding Organization :
Other organizations : Zhujiang Hospital, Southern Medical University, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Fujian Provincial Hospital, The Ohio State University Wexner Medical Center
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Variable analysis
independent variables
- Antibodies used for Western blotting: EPB41L3, caspase-8, caspase-9, caspase-3, CyclinA, CyclinD, CyclinE, CyclinB1, Cyclin-dependent kinase 1 (CDK1, also known as Cdc2)
- Not explicitly mentioned
- GAPDH (1:8,000; ProteinTech Wuhan Sanying Biotechnology) was used as loading control
- Positive control: not specified
- Negative control: not specified
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