Demyelination in mice was induced by injection of 1 µL of 1% L-α-lysophosphatidylcholine (lysolecithin, SIGMA, St. Louis, MI, USA) into the ventral and dorsal white matter funiculi at the level of T12 as previously described in detail [40 (link),41 (link)]. Briefly, the position of T13 was identified and the epaxial musculature was cleared from the immediate area. The space between T12 and T13 was exposed and carefully cleared, the central vein was identified, and the dura was pierced with a dental needle lateral to the vein. A three-way manipulator was then used to position the needle for stereotaxic injection of EB. Hamilton needle with a fine glass tip was advanced through the pierced dura at an angle appropriate for ventrolateral or dorsal funiculus injection. The injection was controlled at 1 µL per minute and the needle remained in the injection site for 2 min to allow maximal diffusion of toxin.
During all surgical procedures, animals were anesthetized with isoflurane supplemented with buprenorphine (0.03 mg/kg, intraperitoneal injection) for pain relief.
During all surgical procedures, animals were anesthetized with isoflurane supplemented with buprenorphine (0.03 mg/kg, intraperitoneal injection) for pain relief.
Free full text: Click here
