The studied compound 2-{5-[4-(2-methoxyphenyl)piperazin-1-ylo]butoxy}benzamide hydrochloride, JJGW07, was synthesized in the Department of Organic Chemistry and Technology, Faculty of Chemical and Engineering and Technology, Cracow University of Technology. The synthesis and biological properties of the compound were described earlier [5 (link)].
JJGW07 was dissolved in saline (0.9 % NaCl, Polpharma, Starogard Gdańsk, Poland) and administered intraperitoneally (ip) 30 min before each behavioral test. The chemicals used in the radioligand studies, i.e., methiothepin (Sigma-Aldrich, Darmstadt, Germany), were dissolved in saline. MK-801 (Sigma-Aldrich, Darmstadt, Germany) was dissolved in saline and administered ip 15 min before experiments, whereas amphetamine (Sigma-Aldrich, Darmstadt, Germany) was dissolved in saline and administered subcutaneously (sc) 30 min before tests. Aripiprazole (Sigma-Aldrich, Darmstadt, Germany) was dissolved in 1% Tween (J.T. Baker, Phillipsburg, NJ, USA) and administered ip 30 min before experiments. The control groups received 0.9% NaCl solution or 1% Tween as a vehicle. The tested compound was administered at the dose range of 0.625–2.5 mg/kg. The dose range was chosen based on our previous experiments on salicylamide derivatives [6 (link)]. If the effect of the lowest dose, i.e., 0.625 mg/kg, was still statistically significant, we decreased the dose by half until antidepressant-like, anxiolytic-like, antipsychotic-like, or anti-amnesic activity disappeared.
The two exceptions were the effect on motor coordination and the cataleptogenic potential of JJGW07, as to perform those experiments, we needed to use higher doses, i.e., 5–40 mg/kg.
JJGW07 was dissolved in saline (0.9 % NaCl, Polpharma, Starogard Gdańsk, Poland) and administered intraperitoneally (ip) 30 min before each behavioral test. The chemicals used in the radioligand studies, i.e., methiothepin (Sigma-Aldrich, Darmstadt, Germany), were dissolved in saline. MK-801 (Sigma-Aldrich, Darmstadt, Germany) was dissolved in saline and administered ip 15 min before experiments, whereas amphetamine (Sigma-Aldrich, Darmstadt, Germany) was dissolved in saline and administered subcutaneously (sc) 30 min before tests. Aripiprazole (Sigma-Aldrich, Darmstadt, Germany) was dissolved in 1% Tween (J.T. Baker, Phillipsburg, NJ, USA) and administered ip 30 min before experiments. The control groups received 0.9% NaCl solution or 1% Tween as a vehicle. The tested compound was administered at the dose range of 0.625–2.5 mg/kg. The dose range was chosen based on our previous experiments on salicylamide derivatives [6 (link)]. If the effect of the lowest dose, i.e., 0.625 mg/kg, was still statistically significant, we decreased the dose by half until antidepressant-like, anxiolytic-like, antipsychotic-like, or anti-amnesic activity disappeared.
The two exceptions were the effect on motor coordination and the cataleptogenic potential of JJGW07, as to perform those experiments, we needed to use higher doses, i.e., 5–40 mg/kg.
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