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Multiplex immunohistochemistry protocol

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Martin J.C., Chang C., Boschetti G., Ungaro R., Giri M., Grout J.A., Gettler K., Chuang L.S., Nayar S., Greenstein A.J., Dubinsky M., Walker L., Leader A., Fine J.S., Whitehurst C.E., Mbow M.L., Kugathasan S., Denson L.A., Hyams J.S., Friedman J.R., Desai P.T., Ko H.M., Laface I., Akturk G., Schadt E.E., Salmon H., Gnjatic S., Rahman A.H., Merad M., Cho J.H, & Kenigsberg E. (2019). Single-Cell Analysis of Crohn’s Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell, 178(6), 1493-1508.e20.
Publication 2019
X0909 Nanozoomer S60 Digital Slide Scanner anti-human CD20 (clone L26, anti-human CD68 (clone KP1,
3 amino 9 ethylcarbazole Anti cd3 Antibodies Antibody Antigen Biologicals Buffer Chromogenic Citrate Clone Dc lamp Embedded paraffin Ethanol Formalin Glycerol Hematoxylin Hla dr Horseradish peroxidase Human Hydrogen peroxide Novus Scanner Serum protein solution Streptavidin Tissue Xylene

Corresponding Organization : Icahn School of Medicine at Mount Sinai

Other organizations : Boehringer Ingelheim (United States), Emory University, University of Cincinnati, Cincinnati Children's Hospital Medical Center, Connecticut Children's Medical Center, Janssen (United States)

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