Western Blot Analysis of Inflammatory Markers

Western blot analysis was done, as explained below [16 (link),22 (link)]. The primary antibodies were treated during 24 hours for TNF-α (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), IL-1β (1:1,000; Santa Cruz Biotechnology), NF-κB (1:1,000; Abcam, Cambridge, UK), IκBα (1:1,000; Santa Cruz Biotechnology), phosphorylated IκBα (p-IκBα) (1:1,000; Santa Cruz Biotechnology), extracellular signal-regulated kinase (ERK) (1:1,000; Cell Signaling Technology), phosphorylated ERK (1:1,000; Cell Signaling Technology), c-Jun N-terminal kinases (JNK) (1:1,000; Cell Signaling Technology), phosphorylated JNK (1:1,000; Cell Signaling Technology), p38 (1:1,000; Cell Signaling Technology), and phosphorylated p38 (1:1,000; Cell Signaling Technology). The secondary antibodies were applied to the membrane, and then the bands were calculated by enhanced chemiluminescent kit.

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Kim S.H., Ko Y.J., Kim J.Y, & Sim Y.J. (2021). Treadmill Running Improves Spatial Learning Memory Through Inactivation of Nuclear Factor Kappa B/Mitogen-Activated Protein Kinase Signaling Pathway in Amyloid-β-Induced Alzheimer Disease Rats. International Neurourology Journal, 25(Suppl 1), S35-43.

Publication 2021

TNF-α IL-1β NF-κB phosphorylated IκBα (p-IκBα) extracellular signal-regulated kinase (ERK) phosphorylated ERK c-Jun N-terminal kinases (JNK) phosphorylated JNK phosphorylated p38

Antibodies C jun n terminal kinases Extracellular signal regulated kinase 1 Il 1β Iκbα Membrane Nf κb Tnf α Western blot analysis

Corresponding Organization :

Other organizations : Sangmyung University, Kangnam University, Korea University, Kunsan National University

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Variable analysis

independent variables

Treatment with primary antibodies against TNF-α, IL-1β, NF-κB, IκBα, phosphorylated IκBα (p-IκBα), extracellular signal-regulated kinase (ERK), phosphorylated ERK, c-Jun N-terminal kinases (JNK), phosphorylated JNK, p38, and phosphorylated p38

dependent variables

Protein expression and phosphorylation levels of TNF-α, IL-1β, NF-κB, IκBα, ERK, JNK, and p38

control variables

Treatment duration of 24 hours for the primary antibodies

controls

No positive or negative controls were explicitly mentioned in the provided information.

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