Trophoblast cell colony analysis

Culture medium of trophoblast cell colony on the fibroblast feeder layer or in the absence of feeder cells was changed to DMEM (+FBS) containing dye-quenched (DQ) collagen (50-fold dilution) (Invitrogen, USA) [14 (link),15 (link)]. After incubation for 2 h, the cell colonies were once washed with DMEM (−FBS). And the medium was changed to DMEM (−FBS) containing 4 to 40 μM of 12-KETE. Chromatic images were observed using a fluorescence microscope (IX70; OLYMPUS, Japan).

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, & Kamada H. (2022). 12-Oxoeicosatetraenoic acid, a candidate signal for placenta separation, activates matrix metalloproteinase and induces apoptosis in bovine trophoblast cells. Animal Bioscience, 36(3), 429-440.

Publication 2022

DQ) collagen

12 kete Cell Collagen Culture cell Dilution Feeder cells Feeder layer Fibroblast Fluorescence microscope Trophoblast

Corresponding Organization : Institute of Livestock and Grassland Science

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Variable analysis

independent variables

Concentration of 12-KETE (4 to 40 μM)

dependent variables

Chromatic images observed using a fluorescence microscope

control variables

Culture medium of trophoblast cell colony on the fibroblast feeder layer or in the absence of feeder cells
Incubation time (2 h)
DQ collagen (50-fold dilution)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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