Colorimetric Viability Assay for SW839 and Caki-2 Cells

MTT colorimetric assay was performed to measure cell viability. First, 5000 cells of SW839 and Caki-2 were planted into 96-well plates. Transfections were performed in groups of experiments or stimulated with AZD6244 for 24 h. Cell medium was discarded and replaced with MTT reagent (Sigma-Aldrich) and serum-free medium in 96-well plates. Then, the 96-well plate was incubated for 3 to 4 h. A microplate reader (Massachusetts, USA) was used to measure the absorbance of each space.

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Zhu M., Zhang R.N., Zhang H., Qu C.B., Zhang X.C., Ren L.X., Yang Z, & Gu J.F. (2023). PCGF6/MAX/KDM5D facilitates MAZ/CDK4 axis expression and pRCC progression by hypomethylation of the DNA promoter. Epigenetics & Chromatin, 16, 9.

Publication 2023

MTT reagent

Assay Azd6244 Cell Cell viability Colorimetric Serum Transfections

Corresponding Organization :

Other organizations : Second Hospital of Hebei Medical University, Hebei Medical University, Xingtai People's Hospital

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Variable analysis

independent variables

Transfections performed in groups of experiments
Stimulation with AZD6244 for 24 h

dependent variables

Cell viability

control variables

Cell line (SW839 and Caki-2)
Initial cell count (5000 cells)
Incubation time (3 to 4 h)
MTT reagent (Sigma-Aldrich)
Serum-free medium
Microplate reader (Massachusetts, USA)

controls

No controls were explicitly mentioned in the input.

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