After preparing paraffin sections, the slides were incubated with a series of primary antibodies, including Iba1, CD86 (Zen-bio, China), and CD206 (Proteintech, China). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, Cell Signaling, USA). Finally, the sections were imaged with a laser confocal microscope (IXplore SPinSR10, Olympus, Japan) and quantified with the ImageJ software. ImageJ circled the region of interest (ROI), counted the co-labeled cells within ROI area, then determined the percentage of co-labeled cells. The result was expressed as a percentage: co-labeled cells (100%) = (total number of double positive cells/total number of Iba1+ cells × 100%).16 (link)