Metabolic Phenotyping of Fam13a Knockout Mice

All animal procedures were approved by Augusta University’s Institutional Animal Care and Use Committee (IACUC). Mice were maintained under standard conditions with controlled 12 h/12 h-light–dark cycle and 21 ± 1 °C room temperature. Lep^ob/ob, Lepr^db/db and C57BL/6 J mice were obtained from Jackson Laboratory (Bar Harbor, ME). Fam13a^−/− mice were backcrossed to C57BL/6 (Stock#: 027, Charles River) for seven generations and genotyped as previously reported [14 (link)]. 4–5 week old Fam13a^+/+ and Fam13a^−/− littermates were fed with high fat diet (HFD, D12492, 60% Kcal from fat, Research Diets, NJ) for up to 12 weeks. Mice were not randomized. Six week old C57BL/6 J male mice were simply randomized into two experimental groups for ad libitum access to low fat control diet (LFD, D12450B, 10% Kcal from fat, Research Diets, NJ) and 60% HFD for up to 12 weeks as reported [16 (link)]. All mice studies were not blinded. All mice were sacrificed after 4 h fasting with isoflurane.