Establishing and Characterizing NPC Cell Lines

HONE1, HK1, CNE1, CNE2, SUNE1, C666-1, and NPC43 NPC cell lines, and immortalized nasopharyngeal epithelial cell lines NP69 and NP460 were cultured as previously described [4 (link),18 (link)]. Cell lines were authenticated by and obtained from the Hong Kong NPC AoE Cell Line Repository. HK11.19 is a HONE1/chromosome 11 microcell hybrid cell line (MCH) that was established by the transfer of an additional intact human chromosome 11 into HONE1 and expressed physiological levels of THY1 (in the recipient HONE1 cells, THY1 expression is downregulated by promoter hymermethylation, and human THY1 maps to chromosome 11q22.3) [4 (link),19 (link)]. Recombinant human PDGF-BB was obtained from PeproTech (PeproTech, Rocky Hill, NJ, USA, #100-14B-10). KX2-391 (Selleckchem, Houston, TX, USA, #S2700), bosutinib (Selleckchem, Houston, TX, USA, #S1014), and saracatinib (Selleckchem, Houston, TX, USA, #1006) were prepared in DMSO at a concentration of 10 mM for an in vitro assay. For the in vivo assay, KX2-391 was first dissolved in DMSO to a concentration of 85 mg/mL, then formulated with PEG-300 (Sigma-Aldrich, St. Louis, MI, USA, #202371) and PBS before injection following the manufacturer’s instructions (the injection solution contains 4% DMSO, 30% PEG-300, and 66% PBS).