Characterizing SERPINE1 in HNSCC cell lines

SERPINE1 expression and proliferation, migration and apoptosis assays were performed using six human head and neck squamous cell carcinoma cell lines (UM-SCC-22A, UM-SCC-22B, UM-SCC-74B, SCC9, SCC25 and FaDu). 293T cells were only used to generate lentivirus-containing supernatants.
UM-SCC-22A, UM-SCC-22B, UM-SCC-74B [58 (link)] and 293T (ATCC^® CRL-3216™; ATCC;http://www.lgcstandards-atcc.org) cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, 100 U/mL streptomycin / penicillin and 2 mM glutamine (Life Technologies Ltd, UK). SCC-9 (ATCC^® CRL-1629™) and SCC-25 (ATCC^® CRL-1628™) HNSCC cell lines were grown in DMEM/F12 (1:1) containing 10% FBS, 100 U/mL streptomycin/penicillin, 2 mM glutamine and 0.4 μg/mL of hydrocortisone. FaDu (ATCC^® HTB-43™) HNSCC cell line from ATCC was grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, 100 U/mL streptomycin/penicillin and 2 mM glutamine. All cell lines were cultured in a humidified atmosphere at 37°C and 5% of CO₂. Cell lines were authenticated comparing the STR profiles obtained using the Cell ID kit (Promega Corporation, Madison, WI) with the original STR profiles previously described (Supplementary files, table S2) [58 (link), 59 (link)].