IGEPAL CA-630 Inactivation of Human CoVs

For the time-course experiments, IGEPAL^® CA-630 (Sigma-Aldrich, Saint Louis, MI, USA, CAS: 9002-93-1) was diluted into a TNE buffer to get 1, 0.2 and 0.08% v/v solutions. This detergent was selected for its non-denaturing properties and well-established protocol for inactivating human CoVs [50 (link)]. Ethanol (Ethanol absolute pure, PanReac AppliChem, CAS: 64-17-5) was mixed with TNE 10× buffer to get a TNE 1× 60% ethanol v/v buffer. Gradients were performed with Genie Syringe Pump (Kent Scientific, Torrington, CT, USA).

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Cantero M., Carlero D., Chichón F.J., Martín-Benito J, & De Pablo P.J. (2022). Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments. Cells, 11(11), 1759.

Publication 2022

IGEPAL® CA-630 Ethanol absolute pure

Buffer Detergent Ethanol Genie Human Igepal ca 630 Syringe

Corresponding Organization : Centro Nacional de Biotecnología

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Variable analysis

independent variables

IGEPAL® CA-630 concentration (1%, 0.2%, 0.08% v/v)

dependent variables

Not explicitly mentioned

control variables

TNE buffer
Ethanol concentration (60% v/v in TNE 1x buffer)
Gradient formation using Genie Syringe Pump

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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