Diverse Antibody and Reagent Sources

PHF1 (phospho-Ser396/404) and 12E8 (phospho-Ser262) antibodies were kindly provided by Dr. P. Davies and Dr. P. Seubert, respectively. Anti-tau polyclonal (A0024) antibody was purchased from Dako. Anti-Nrf2 (12721), TFEB (4220), mTOR (2983), pmTOR (phospho-Ser2448, 5536), p70S6K (2708), pp70S6K (phospho-Thr389, 9234), 4E-BP1 (9644), p4E-BP1 (phospho-Thr37/46, 2855), NDP52 (9036) and HA (2367) antibodies were purchased from Cell Signaling Technology. Anti-LC3 antibody (PD014) was obtained from Medical & Biological Laboratories. Anti-lamin (A/C) (SC-6215) and β-actin (MAB1501) antibodies were purchased from Santa Cruz Biotechnology and Millipore, respectively. Anti-heme oxygenase (HO)-1 (ADI-SPA-895) and p62/SQSTM1 (BML-PW9860) antibodies were obtained from Enzo Life Sciences. Anti-ATG9b (PA5-20998) antibody was purchased from Thermo Fisher Scientific. Anti-tubulin (T6074) antibody was obtained from Sigma. The GFP-LC3 plasmid was used previously18 (link). The GSK-3β-S9A plasmid was kindly provided by Dr. E. Choi. The mouse TFEB specific predesigned siRNA (S74859) was purchased from Life Technology. The ON-TARGET plus mouse Nrf2 (18024) siRNA SMART pool was purchased from GE Healthcare. Fisetin (5016) was purchased from Tocris. Chemicals such as bafilomycin A1 (B1793) and chloroquine (C6628) were obtained from Sigma.

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Kim S., Choi K.J., Cho S.J., Yun S.M., Jeon J.P., Koh Y.H., Song J., Johnson G.V, & Jo C. (2016). Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors. Scientific Reports, 6, 24933.

Publication 2016

HA (2367) Anti-lamin (A/C) (SC-6215) Anti-heme oxygenase (HO)-1 (ADI-SPA-895) bafilomycin A1 (B1793) chloroquine (C6628)

4e bp1 Actin Anti antibody Antibodies Antibody Bafilomycin a1 Biological Chloroquine Fisetin Heme oxygenase 1 Lamin a c Mouse Mtor Nrf2 P70s6k Phf1 Plasmid Sirna Tfeb Tubulin

Corresponding Organization :

Other organizations : Korea National Institute of Health, University of Rochester Medical Center

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Fisetin (5016)
Bafilomycin A1 (B1793)
Chloroquine (C6628)
GSK-3β-S9A plasmid
Mouse TFEB specific predesigned siRNA (S74859)
ON-TARGET plus mouse Nrf2 (18024) siRNA SMART pool

dependent variables

Phosphorylation of tau at Ser396/404 (PHF1 antibody)
Phosphorylation of tau at Ser262 (12E8 antibody)
Levels of tau (anti-tau polyclonal (A0024) antibody)
Levels of Nrf2 (anti-Nrf2 (12721) antibody)
Levels of TFEB (anti-TFEB (4220) antibody)
Levels of mTOR (anti-mTOR (2983) antibody)
Phosphorylation of mTOR at Ser2448 (anti-pmTOR (phospho-Ser2448, 5536) antibody)
Levels of p70S6K (anti-p70S6K (2708) antibody)
Phosphorylation of p70S6K at Thr389 (anti-pp70S6K (phospho-Thr389, 9234) antibody)
Levels of 4E-BP1 (anti-4E-BP1 (9644) antibody)
Phosphorylation of 4E-BP1 at Thr37/46 (anti-p4E-BP1 (phospho-Thr37/46, 2855) antibody)
Levels of NDP52 (anti-NDP52 (9036) antibody)
Levels of HA (anti-HA (2367) antibody)
Levels of LC3 (anti-LC3 antibody (PD014))
Levels of lamin (A/C) (anti-lamin (A/C) (SC-6215) antibody)
Levels of β-actin (anti-β-actin (MAB1501) antibody)
Levels of heme oxygenase (HO)-1 (anti-HO-1 (ADI-SPA-895) antibody)
Levels of p62/SQSTM1 (anti-p62/SQSTM1 (BML-PW9860) antibody)
Levels of ATG9b (anti-ATG9b (PA5-20998) antibody)
Levels of tubulin (anti-tubulin (T6074) antibody)

control variables

GFP-LC3 plasmid

Annotations

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