HBV Infection of PHH/NIH3T3 Co-culture

PHH were seeded at a density of 30,000 cells/well in collagen-coated 96-well plates (Corning) in WEM supplemented with Thawing/Plating Supplement Pack (Invitrogen). After 24 h, 15,000 NIH3T3/J2 cells in WEM supplemented with Cell Maintenance Supplement Pack (Invitrogen) were added and the medium was changed every 48 h for 10 days before infection with HBV. DMSO 0.5% was added to the cultures 24 h before HBV infection as previously described^{19 (link)}.

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Ortega-Prieto A.M., Skelton J.K., Wai S.N., Large E., Lussignol M., Vizcay-Barrena G., Hughes D., Fleck R.A., Thursz M., Catanese M.T, & Dorner M. (2018). 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection. Nature Communications, 9, 682.

Publication 2018

collagen-coated 96-well plates Thawing/Plating Supplement Pack Cell Maintenance Supplement Pack

Cell Collagen Dmso Infection Nih3t3 cells Supplement

Corresponding Organization :

Other organizations : Imperial College London, CN Bio Innovations (United Kingdom), King's College London

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Variable analysis

independent variables

Density of PHH cells seeded (30,000 cells/well)
Addition of NIH3T3/J2 cells (15,000 cells)
Addition of DMSO (0.5%) 24 h before HBV infection

dependent variables

HBV infection

control variables

Collagen-coated 96-well plates
WEM supplemented with Thawing/Plating Supplement Pack (Invitrogen)
WEM supplemented with Cell Maintenance Supplement Pack (Invitrogen)
Medium change every 48 h for 10 days before infection

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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