Measuring Intracellular Calcium Levels
Corresponding Organization :
Other organizations : Augusta University, Nanjing Medical University, University of Giessen
Variable analysis
- Different concentrations of PLY
- Intracellular calcium levels measured using calcium indicator Fluo-4 AM or calcium-activated photoprotein aequorin
- COS-7, HULEC5α or Cav-1 KO HULEC5α cells transduced with adenoviruses encoding aequorin, RFP or Cav-1 at 15 MOI
- Aequorin reconstitution by treating cells with 5 μM coelenterazine for 1 hour in serum-free and phenol free DMEM containing 0.1 mM EDTA
- Calcium chloride injection at a 1.8 mM final concentration to enable the measurement of calcium induced changes in luminescence which reflect calcium influx
- Not explicitly mentioned
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