Virus particles were harvested based on instructions of the SBI packaging protocol of Lenti-Concentin Virus Precipitation Solution (Cat No. LV810A-1). Both cells DU145 and LNCap were infected using the TransDux virus transduction reagent (Cat No. LV850A-1) (SBI, USA). The following isolation used a flow cytometer, and the infected cells were cultured in 96-well plates [21 (link)].
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