Lentiviral Transduction and Flow Cytometry
Corresponding Organization : University of California, Riverside
Variable analysis
- Type of cell line used: DU145 and LNCap
- Infected cells cultured in 96-well plates
- Virus particles harvested based on instructions of the SBI packaging protocol of Lenti-Concentin Virus Precipitation Solution (Cat No. LV810A-1)
- Cells infected using the TransDux virus transduction reagent (Cat No. LV850A-1) (SBI, USA)
- Flow cytometer used for isolation of infected cells
- No positive or negative controls were explicitly mentioned in the provided information.
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