Acute Peritonitis Model for Immune Cell Tracking

The acute peritonitis model was generated as described [7 (link)]. In brief, C57BL/6 wild-type mice (recipient mice) were given intraperitoneal injection of 5% thioglycollate (1 ml/mouse) to induce acute peritonitis. 24 h later, the recipient mice were exposed to total-body X-ray irradiation at a dose of 6 Gy. And then, carboxyfluorescein succinimidyl ester (CFSE)-labeled bone marrow cells (2 × 10⁷ per mouse) from PRL2^−/− CD45.2⁺ and wild-type CD45.1⁺ mice, mixed at 1:1 ratio were injected into peritoneal cavity of recipient mice. Recipient mice were sacrificed 24 h later and their peritoneal cells were collected. The peritoneal cells were stained with anti-CD45.1-PE (eBioscience, USA), anti-CD45.2-PerCP/Cy5.5 (eBioscience, USA) and anti-CD11b-APC (eBioscience, USA), and the frequencies of PRL2^−/−CD45.2⁺CFSE⁺CD11b⁺ and PRL2^+/+CD45.1⁺CFSE⁺CD11b⁺ cells were determined by flow cytometry.