Targeting Senescent Cells in Aging Mice

All animal protocols were approved by the Institutional Animal Care and Use Committee (IACUC), and all experiments were performed in accordance with IACUC guidelines. Mice were housed in ventilated cages in a pathogen-free facility (12-hour light/dark cycle, 23 °C) and had access to food (standard mouse diet, Lab Diet 5053, St. Louis, MO) and water ad libitum. Mouse experiments for a genetic targeting approach of senescent cells have been described by our group earlier^{37 (link)}. Briefly, old (20 months) female mice were injected intraperitoneally with vehicle (4% of 100% EtOH, 10%PEG400, 86% of 2% Tween 20 in deionized Water) or AP20187 (B/B homodimerizer, Clontech; 10 mg of AP20187 per kg body mass) twice weekly at the age of 20 months for a total of 4 months (old mice were sacrificed at 24 months of age). In addition, young (6-month) INK-ATTAC mice were used as a control comparison cohort.

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Saul D., Kosinsky R.L., Atkinson E.J., Doolittle M.L., Zhang X., LeBrasseur N.K., Pignolo R.J., Robbins P.D., Niedernhofer L.J., Ikeno Y., Jurk D., Passos J.F., Hickson L.J., Xue A., Monroe D.G., Tchkonia T., Kirkland J.L., Farr J.N, & Khosla S. (2022). A new gene set identifies senescent cells and predicts senescence-associated pathways across tissues. Nature Communications, 13, 4827.

Publication 2022

B/B homodimerizer AP20187

Animal Ap20187 Body Cells senescent Diet Etoh Female Food Institutional animal care and use committee Mice Pathogen Peg400 Tween 20

Corresponding Organization : Mayo Clinic in Arizona

Other organizations : Mayo Clinic in Florida, University of Minnesota, The University of Texas at San Antonio, The University of Texas Health Science Center at San Antonio

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Variable analysis

independent variables

Injection of vehicle (4% of 100% EtOH, 10%PEG400, 86% of 2% Tween 20 in deionized Water)
Injection of AP20187 (B/B homodimerizer, Clontech; 10 mg of AP20187 per kg body mass)

dependent variables

Not explicitly mentioned

control variables

Mouse age (20 months old for old mice, 6 months old for young mice)
Mouse strain (INK-ATTAC mice)
Housing conditions (ventilated cages in a pathogen-free facility, 12-hour light/dark cycle, 23 °C)
Food and water access (standard mouse diet, Lab Diet 5053, St. Louis, MO, and water ad libitum)

controls

Positive control: Young (6-month) INK-ATTAC mice
Negative control: Vehicle (4% of 100% EtOH, 10%PEG400, 86% of 2% Tween 20 in deionized Water) injection

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