Protein Expression Profiling of Vascular Cells

Aliquots of protein extracts (20 μg) derived from THP1 monocytes, their derived macrophages, HUVECs, and HASMCs were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and then immunoblotted with specific antibodies raised against the following proteins: CD68, ACAT1, ICAM1, VCAM1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), ABCA1, collagen 1 (Novus Biologicals, Littleton, CO, USA), collagen 3, fibronectin, arginase 1, phospho (Ser529)-NFκB, α-tubulin, MMP2 (GeneTex, Irvine, CA, USA), MMP9 (EnoGene, Atlanta, GA, USA), elastin, MARCO, selectin E (Bioss, Woburn, MA, USA), PPARγ (Signalway Antibody, College Park, MD, USA), phospho (Thr202/Tyr204)-ERK1/2, phospho (Ser/Thr)-Akt (Cell Signaling Technology, Tokyo, Japan), c-Src (Bioworld Technology, St. Louis Park, MN, USA), PI3K, Bcl2, Bax, and β-actin (Sigma). The band intensity of the immunoblot was quantified by densitometry [39 (link),40 (link),41 (link),44 (link),45 (link),46 (link),47 (link),48 (link),49 (link)].

Free full text: Click here

Sato K., Yamashita T., Shirai R., Shibata K., Okano T., Yamaguchi M., Mori Y., Hirano T, & Watanabe T. (2018). Adropin Contributes to Anti-Atherosclerosis by Suppressing Monocyte-Endothelial Cell Adhesion and Smooth Muscle Cell Proliferation. International Journal of Molecular Sciences, 19(5), 1293.

Publication 2018

ACAT1 ICAM1 VCAM1 ABCA1 collagen 1 collagen 3 fibronectin arginase 1 α-tubulin elastin PPARγ phospho (Thr202/Tyr204)-ERK1/2 c-Src Bcl2 Bax β-actin

Abca1 Actin Antibodies Antibody Arginase 1 Bcl2 Biologicals C src Collagen Collagen 1 Densitometry Elastin Erk1 Fibronectin Icam1 Immunoblot Macrophages Mmp2 Mmp9 Monocytes Nfκb Novus Pi3k Pparγ Proteins Selectin e Sodium dodecyl sulfate polyacrylamide gel electrophoresis α tubulin

Corresponding Organization : Tokyo University of Pharmacy and Life Sciences

Other organizations : Showa University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Protein extracts derived from THP1 monocytes, their derived macrophages, HUVECs, and HASMCs

dependent variables

Band intensity of the immunoblot quantified by densitometry for the following proteins: CD68, ACAT1, ICAM1, VCAM1, ABCA1, collagen 1, collagen 3, fibronectin, arginase 1, phospho (Ser529)-NFκB, α-tubulin, MMP2, MMP9, elastin, MARCO, selectin E, PPARγ, phospho (Thr202/Tyr204)-ERK1/2, phospho (Ser/Thr)-Akt, c-Src, PI3K, Bcl2, Bax, and β-actin

control variables

Amount of protein extracts (20 μg) loaded onto the 10% sodium dodecyl sulfate polyacrylamide gel for electrophoresis

controls

Positive controls: None specified
Negative controls: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!