Induction and Maintenance of iPSC

An overview of all lines is present in Table S4 (Online Resource 17). The CV-B iPSC-line has been described previously [18 (link)]. The study approval for Ctrl-1-SC11 was granted by the local ethics committee (No. 4485, FAU Erlangen-Nuernberg, Germany). IPSC were cultured on Matrigel coated dishes and fed all 24 h with mTesR1 (Stemcell Technologies). Upon reaching ~ 80% confluency, iPSC were passaged using Accutase in a 1:3–1:6 ratio. After passaging and thawing, mTesR1 was supplemented with 10 µM Y27632 (ROCK-inhibitor, RI, Tocris).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Krach F., Batra R., Wheeler E.C., Vu A.Q., Wang R., Hutt K., Rabin S.J., Baughn M.W., Libby R.T., Diaz-Garcia S., Stauffer J., Pirie E., Saberi S., Rodriguez M., Madrigal A.A., Kohl Z., Winner B., Yeo G.W, & Ravits J. (2018). Transcriptome–pathology correlation identifies interplay between TDP-43 and the expression of its kinase CK1E in sporadic ALS. Acta Neuropathologica, 136(3), 405-423.

Publication 2018

mTesR1 Y27632

Accutase Dishes Ipsc Local ethics committee Matrigel Stemcell Y27632

Corresponding Organization : National University of Singapore

Other organizations : University of California, San Diego, Behavioral Tech Research, Inc., Friedrich-Alexander-Universität Erlangen-Nürnberg, Universitätsklinikum Erlangen

Top 5 similar protocols

Variable analysis

independent variables

Passaging of iPSC using Accutase in a 1:3–1:6 ratio
Supplementation of mTesR1 with 10 µM Y27632 (ROCK-inhibitor, RI, Tocris) after passaging and thawing

dependent variables

Confluency of iPSC cultures

control variables

Culturing of iPSC on Matrigel coated dishes
Feeding of iPSC with mTesR1 every 24 h

controls

Positive control: CV-B iPSC-line, previously described [18]
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!