Culturing and Extracting R. solanacearum FJ1003

The highly pathogenic R. solanacearum strain “FJ1003” (Zhang et al., 2017 (link); Zhang et al., 2019 (link)) was provided by Professor Liu Bo from the Fujian Academy of Agricultural Sciences, Fuzhou, China (isolated from tobacco growing areas in Fujian Province). The strain was cultured on the bacteria peptone glucose (BG) solid medium (peptone 1.0%, yeast extract 0.1%, casamino acid 0.1%, glucose 1.0%, agar 1.4% and pH 7.4), and 50 ml BG liquid medium (peptone 1.0%, yeast extract 0.1%, casamino acid 0.1%, glucose 1.0%, pH 7.4) was used for single clone culturing at 28°C, 200 rpm overnight. The bacterial genome extraction kit (TIANGEN, Beijing, China) was used to extract the genomic DNA from the bacterial culture solution of R. solanacearum for subsequent sequencing experiments.