A lentiviral (pLV) plasmid expressing the PIP-FUCCI fluorescent indicator protein construct was obtained from VectorBuilder (27 (link)). Lentivirus was prepared as described in previous publications and fluorescent cells were isolated using flow cytometry (Becton Dickinson FACS Aria; ref. 18 (link)). Cells were imaged using an EVOS M5000 fluorescent microscope (Thermo Fisher Scientific).
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