Immunohistochemical Staining of CD8A and PD-L1

Paraffin‐embedded tissue sections were incubated for 120 min at 37°C with the primary antibodies anti‐CD8A (1:2000 dilution; Cat#ab217344; Abcam, USA) and PD‐L1 (1:5000 dilution; Cat#66248‐1‐Ig; Proteintech, Wuhan, China). Following this, HRP‐conjugated secondary antibodies were applied and incubated for 30 minutes at the same temperature. The sections were then stained with DAB (3,3′‐diaminobenzidine) and counterstained with haematoxylin
³² (link) for visualization.

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Zhang P., Pei S., Zhou G., Zhang M., Zhang L, & Zhang Z. (2024). Purine metabolism in lung adenocarcinoma: A single‐cell analysis revealing prognostic and immunotherapeutic insights. Journal of Cellular and Molecular Medicine, 28(8), e18284.

Publication 2024

anti‐CD8A ab217344 PD‐L1

Corresponding Organization : Tianjin Medical University Cancer Institute and Hospital

Other organizations : Nanjing Medical University, Jiangsu Province Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Incubation time (120 min)
Incubation temperature (37°C)
Primary antibodies (anti-CD8A, PD-L1)

dependent variables

Staining intensity/pattern of CD8A and PD-L1

control variables

Paraffin-embedded tissue sections
HRP-conjugated secondary antibodies
DAB staining
Haematoxylin counterstaining

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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