ATAC-seq on Live Cells
Corresponding Organization : University of Oxford
Other organizations : Korea Advanced Institute of Science and Technology, University of Michigan–Ann Arbor, University of Pennsylvania, Rockefeller University, University of California, San Francisco
Variable analysis
- The use of Nextera Tn5 transposase (Illumina) to conduct ATAC-seq on the cells
- The sequencing of the libraries by paired-end sequencing with a 75-cycle high-output Nextseq 500 kit (Illumina)
- The number of live cells used (5 × 10^4)
- No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!