For PCR, genomic DNA was extracted from conidia with the DNeasy 96 Plant Kit (Qiagen) as already described (Daverdin et al., 2012 (link)). Standard PCRs were performed in an Mastercycler EP gradient thermocycler (Eppendorf) as described in Daverdin et al. (2012 (link)). Sequencing was performed using a CEQ 8000 automated sequencer (Beckman Coulter) according to the manufacturer's instructions.
Total RNA was extracted from infected cotyledons of B. napus or B. oleracea genotypes using TRIzol reagent (Invitrogen) according to manufacturer's protocol. Total RNA was treated with RNase‐free DNase I (New England Biolabs).
Total RNA was extracted from infected cotyledons of B. napus or B. oleracea genotypes using TRIzol reagent (Invitrogen) according to manufacturer's protocol. Total RNA was treated with RNase‐free DNase I (New England Biolabs).
