Mouse Brain Fatty Acid Analysis

Fatty acid profiles of mouse brain tissues were analyzed using gas chromatography as described previously^{79 (link)}. Briefly, tissues were homogenized by grinding in liquid nitrogen, and subjected to fatty acid methylation by mixing with 1 ml of hexane and 1 ml of 14% BF3/MeOH reagent at 100 °C for 1 h. Fatty acid methyl esters were extracted in the hexane phase, and then the fatty acid profiles were analyzed using a fully automated HP6890 gas chromatography system equipped with a flame-ionization detector (Agilent Technologies, Palo Alto, CA, USA). The fatty acid peaks were identified by comparing relative retention times with commercial mixed standards (Nu-Chek Prep, Elysian, MN, USA), and the area and its percentage for each peak were analyzed using GC Chemstation software. Data were analyzed using a two-tailed Student’s t-test.